Design
Score Constructs Before You Clone
You design a bunch of constructs, commit them to the bench, and wait weeks to learn which ones were never going to express. Design scores every candidate in silico and surfaces the handful worth making.
Yield Ranking, with a Developability Read
Yield is the strong, validated signal — the axis that decides whether a construct is worth making. Developability rides alongside it as an early risk read, calibrated on real mammalian constructs.
See It in the Workspace
From a Sequence to a Ranked Shortlist
Score and pick constructs in the same tool you characterize and stabilize in — with the yield and developability read side by side.
Tumor Necrosis Factor
| Method | Host | Construct | Yield | Developability | |
|---|---|---|---|---|---|
| Reference | Cell Free | N Target C | 41.2 | 50.0 | |
| Combinatorial | Cell Free | N SUMO MBP HRV 3C Target (80-227) C | 91.2 | 63.1 | |
| Combinatorial | Cell Free | N SUMO MBP HRV 3C His8 Target (80-233) C | 86.5 | 68.9 | |
| Combinatorial | Cell Free | N SUMO MBP HRV 3C Target (80-233) Strep-tag II C | 84.0 | 68.9 | |
| Combinatorial | Cell Free | N SUMO MBP HRV 3C His8 Target (80-233) C | 82.7 | 68.9 | |
| Combinatorial | Cell Free | N SUMO MBP HRV 3C His8 Target (80-227) C | 79.3 | 63.1 | |
| Combinatorial | Cell Free | N SUMO MBP HRV 3C His6 Target (80-227) C | 73.5 | 63.1 | |
| Combinatorial | Cell Free | N SUMO MBP HRV 3C His6 Target (80-233) His6 C | 61.8 | 68.9 | |
| Combinatorial | Cell Free | N SUMO MBP HRV 3C His8 Target (80-233) Strep-tag II C | 44.2 | 68.9 |
Two Scores, One Decision
Rank for Yield, Watch the Trade-offs
Tune for what the project actually needs — and see the trade-offs before you commit a single clone.
Expression Yield
Every construct is ranked by predicted expression yield — the read that decides whether it's worth making at all. Validated prospectively and blind on cell-free panels.
Developability
An early flag on the liabilities that sink a construct downstream — solubility, aggregation propensity, and PTM liabilities — so the risky ones surface before you commit a plate.
A Real Trade-off, Confirmed at the Bench
Design kept disordered C-terminal residues most people trim — trading a little yield for better developability. Predicted and measured agreed on the direction of both axes.
Read the Resultsvertical mark = common reference construct
In Detail
Everything Design Scores and Searches
A construct is a set of choices — boundaries, tags, fusions, host. Design scores all of them, and shows you the search space it covered.
Expression Yield
- Every Construct Ranked on a 0–100 Scale
- Reference and Combinatorial Designs Side by Side
- Cell-Free and Mammalian Expression Hosts
- Thousands of Candidates Ranked in One Pass
Developability
- Solubility
- Aggregation Propensity
- PTM Liabilities
- An Early Risk Read Carried Alongside Yield
Construct Search
- Truncations and Domain Boundaries
- N- and C-Terminal Tag Placement
- Cleavage-Site Selection
- Position-Aware Scoring, Not a Flat Checklist
Tags & Fusions
- Solubility Fusions — MBP, SUMO
- Affinity Tags — His6 / His8, Strep-tag II
- Cleavage Sites — HRV 3C, TEV
- Every Combination Scored, Not Just the Defaults
Compare & Decide
- Absolute and Comparison Modes
- Star and Shortlist the Candidates Worth Making
- Search by ID, Tag, or Fusion
- Export the Ranked Table
Proof on Your Targets
- A Prospective, Blind Benchmark per Engagement
- Predicted versus Measured on Your Own Data
- Direction-of-Effect Reported Honestly, Either Way
Yield is the validated axis. Developability is an early risk read — useful for triage, not a guarantee.
Design Your Constructs
Score every candidate for yield and developability in silico, and take only the top-ranked few to the bench — instead of filling plates to find the one that expresses.