Platform

Characterize

Everything a Sequence Can Tell You

Paste a sequence and get the full read on a protein — structure and confidence, modifications, binding pockets, membrane topology, and disorder — before you order a single primer.

Structure & pLDDTPTM SitesBinding PocketsTopologyDisorderFunction
Rhodopsin · 7-TM GPCR · Colored N → C Terminus
Signature Strength

One Pass, the Whole Picture

Structure models stop at the fold. Characterize combines structure with the annotations that decide whether a protein is workable — modifications, binding sites, topology, and disorder — in a single run, on any protein family.

39
PTM Types Predicted from Sequence

See It on a Real Protein

Rhodopsin, Fully Annotated

A per-residue read-out: pLDDT confidence, PTM marks, binding sites, and membrane topology — all aligned to the sequence and mapped onto the structure.

Overview
Characterize
Stabilize
Design
Bench
Rhodopsin · OPSD
UniProt P08100·348 aa·7 TM Helices
Analysis Complete
Mean pLDDT
78.3
Disorder Rate
12.5%
TM Helices
7
Binding Pockets
2
Residue-Level Annotations
2D3DTrackSchematic
Index
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
Residue
M
N
G
T
E
G
P
N
F
Y
V
P
F
S
N
K
T
G
V
V
R
S
pLDDT
57
58
50
52
57
55
51
54
59
62
66
69
71
74
78
80
83
86
88
90
92
94
PTMs
Phosphorylation2
P
P
Disulfide Bond2
S
S
N-Glycosylation1
G
Binding & Topology
Binding Sites4
Topology
HighMedLowExtra 71TM 156Intra 110
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PHODISN-GLY

In Detail

Every Annotation Characterize Returns

The full surface area of one run, per residue and across the whole sequence — so you can judge the fit before you talk to us.

Structure Prediction

  • AlphaFold2
  • Boltz-2
  • ESMFold
  • Predicted 3D Structure from Sequence Alone
  • 2D Grid, 3D, Track, and Schematic Views

Structure & Confidence

  • Per-Residue pLDDT Confidence, Colour-Coded
  • Confidence Bands Across the Whole Chain
  • Low-Confidence Regions Flagged, Not Hidden
  • Mean pLDDT and Disorder Rate at a Glance

Post-Translational Modifications

  • Phosphorylation Sites
  • Disulfide Bonds
  • Acetylation and Ubiquitination
  • Oxidation
  • 39 Modification Types in Total

Binding Sites & Cofactors

  • Ligand-Binding Residues, Position by Position
  • Small-Molecule Pockets, Grouped and Ranked
  • Metal Cofactors — Magnesium, Zinc, Nickel, Cobalt
  • Cross-Links Out to the Reference Databases

Membrane Topology

  • Transmembrane Helices and Span Count
  • Extramembrane Regions
  • Inside / Outside Orientation
  • Ambiguous Stretches Called Out Explicitly

Disorder & Aggregation

  • Intrinsically Disordered Regions
  • Amyloid-Aggregation Propensity
  • Read per Residue Along the Full Sequence
  • Aggregation-Prone Stretches Flagged Early

Function & Suitability

  • EC Number and GO Terms
  • Pathways and Protein Categories
  • Cofactors, Domains, and Host Lineage
  • Subcellular Localization

Every annotation is exportable per residue. Exploratory Mode surfaces lower-confidence calls rather than suppressing them.

Characterize Your Target

Bring a sequence and get the full read-out in one pass — structure, modifications, binding, topology, and disorder. No wet lab required to know where to look first.