Stabilize
Find the Mutations That Hold
Stabilizing a membrane target usually means screening dozens of mutants for a couple of usable hits. Stabilize ranks point mutations by predicted stability gain, so the variants worth making rise to the top.
A Directional Pre-Screen for Stability
On our hardest target class — GPCRs — Stabilize called the direction of a mutation's effect right most of the time, across 190 mutations in 7 public receptors. The signal is in the ranking: fewer rounds to a usable, stabilizing hit.
See It in the Workspace
Every Variant, Scored and Reviewable
Each candidate arrives with its stability scores, its structural cost, and its liability deltas — then you accept, reject, or bookmark it before anything reaches the bench.
Adenosine A2A Receptor
| Variant | Status | Changes | ΔTm | ΔΔG | Δ pLDDT | TM RMSD | Pocket RMSD | Δ Binding |
|---|---|---|---|---|---|---|---|---|
| A112V | Completed | 1 MUT | +4.6 °C | -0.85 kcal/mol | -0.6 | 0.5 Å | 0.4 Å | 100% |
| M74I | Completed | 1 MUT | +3.1 °C | -0.42 kcal/mol | -1.2 | 0.6 Å | 0.5 Å | 100% |
| T203S | Completed | 1 MUT | +2.4 °C | -0.20 kcal/mol | -0.3 | 0.4 Å | 0.3 Å | 100% |
| G88A | Completed | 1 MUT | +0.9 °C | +0.11 kcal/mol | -2.1 | 0.7 Å | 0.6 Å | 100% |
| K41R | Completed | 1 MUT | -1.4 °C | +0.68 kcal/mol | -4.8 | 1.1 Å | 0.9 Å | 96% |
| Δ180-206 | Completed | DELETION | -5.2 °C | +2.31 kcal/mol | -28.4 | 3.8 Å | N/A | 42% |
How It Works
From Every Possible Mutation to a Short, Ranked List
Instead of throwing a screen at every position, take a directional approach — rank variants by predicted stability gain so more of what reaches the bench actually holds.
Enumerate
Scan the sequence for candidate point mutations — or bring your own shortlist.
Predict
Each variant is scored for predicted ΔTm and ΔΔG against the wild type.
Rank
Variants sort by predicted stability gain, with a hotspot map of the best positions.
Ranked, Not Just Scored
Absolute ΔTm values are hard — so we lead with the ranking. The top of the list is where the stabilizing mutations concentrate, and where a couple of bench tests go furthest. Multi-point combinations are supported, but we start from the singles.
Read the GPCR WhitepaperIn Detail
Everything Scored on Every Variant
A stability call is only trustworthy next to what it costs. Here is the full read-out Stabilize returns for each candidate.
Stability Scores
- Predicted ΔTm (°C) Against the Wild Type
- Predicted ΔΔG (kcal/mol)
- Direction and Magnitude, per Variant
- A Ranked Shortlist, Not Just Raw Scores
Structural Cost
- Δ pLDDT — Wild Type versus Variant
- TM-Bundle RMSD
- Binding-Pocket RMSD
- Δ Binding — Share of Binding Sites Preserved
Liability Deltas
- Δ Solubility
- Δ Disorder
- Δ Amyloidogenicity
- PTM Sites Gained or Lost
Variant Types
- Single-Point Mutations
- Multi-Point Combinations
- Deletions and Truncations
- Region Context — Loop, Helix, Transmembrane
Overall Profile
- One Verdict per Variant, Across All Metrics
- A Plain-Language Read on Each Metric
- Borderline Calls Named as Borderline
- Missing Metrics Reported as N/A, Never Guessed
Review Workflow
- Accept, Reject, or Bookmark Each Candidate
- Search and Filter the Candidate Set
- Bring Your Own Shortlist, or Let Stabilize Enumerate
- Export the Ranked Table
We lead with the ranking rather than absolute ΔTm — magnitudes are the hardest part of the problem, and we say so.
Under the Hood
Powered by the Astra Model Suite
Each engine runs on open, benchmarked models — documented in their own preprints and whitepapers. Read the science behind the predictions.
Rank Your Mutations
Bring a target and a set of positions — or let Stabilize scan them — and get a ranked shortlist of the mutations most likely to hold, before you screen a single one.