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Bench Update: Constructs That Come with Reasons and DNA

Mar 3, 2026 · 2 min read

A few weeks ago, getting from "I want a His-tagged truncation of my target" to an orderable gene meant three tools, two hours, and a spreadsheet.

Orbion could give you the construct idea — but you still had to look up the tag sequence, pick linkers, assemble everything, run codon optimization, and sanity-check whether the boundaries made sense structurally.

That workflow was slow, brittle, and frankly not how protein work should feel in 2026.

Bench now takes you from target → construct → synthesis-ready DNA in one session.

Go from target to orderable sequence without leaving the platform

  • Assembled protein + codon-optimized DNA for every construct
  • Tags, linkers, cleavage sites, and fusions resolved to real sequences
  • Boundaries annotated and exportable

Know why a construct was designed the way it was

  • Boundary decisions tied to pLDDT, disorder, PTMs, and binding sites
  • Not "standard domain boundary from literature," but "truncation at residue 85 where pLDDT drops below 70, preserving the predicted binding site at 25–40"
  • You control the risk level: conservative designs that match published approaches, or exploratory ones that push toward non-obvious combinations

Compare constructs before committing to the lab

  • Scores for boundaries, PTMs, binding, domains, and host fit
  • Side-by-side comparison to narrow candidates fast
  • Less brute-force screening, more intentional selection

Know what to expect from your protocol before you start

  • Timeline expectations with per-step durations
  • QC checkpoints with measurable go/no-go criteria
  • Yield expectations when supported

If you're starting a new target — or revisiting one that gave you trouble — run it through the updated Bench. Open your dashboard to try it on your own protein.