Bench
From Picks to Protocol
The in-silico work only pays off if it makes it into the lab. Bench turns your top-ranked constructs and mutations into a literature-grounded, step-by-step protocol — adapted to your target and host.
A Protocol, Not a Template
Bench starts from your ranked pick, searches what has actually worked on similar targets and hosts, and writes a runnable protocol around it — vector, strain, construct layout, and expected outcomes included.
See It in the Workspace
From a Ranked Pick to a Runnable Protocol
Each stage arrives with its construct design, its expected outcomes, and its sources — and waits for your approval before the next one opens.
Tumor Necrosis Factor
Expression
- • The expressed target region is residues 81–233 only; the native transmembrane segment and proximal stalk are intentionally excluded from the expression design.
- • The construct is optimized around a solubility fusion and a prediction-guided domain boundary.
- 1TransformationTransform BL21(DE3) with the expression vector; plate on LB agar + 100 µg/mL ampicillin, 37 °C overnight.
- 2Pre-CultureInoculate a single colony into 5 mL LB + antibiotic; 37 °C, 220 rpm, overnight.
- 3Expression CultureDilute 1:100 into 1 L Terrific Broth; grow at 37 °C to OD600 0.6–0.8.
- 4InductionCool to 18 °C, induce with 0.3 mM IPTG, express 16–20 h at 220 rpm.
- 5HarvestPellet at 5,000 × g for 15 min; flash-freeze and store at −80 °C.
How It Works
The Last Mile, from Prediction to Protocol
A ranked construct is only useful if you can make it. Bench closes the loop — grounding every step in the literature and adapting it to your target.
Start from a Pick
Carry the top-ranked construct or variant straight from Design and Stabilize into a protocol.
Ground in Literature
Bench searches the literature for what has worked on similar targets and hosts — and cites it.
Adapt the Protocol
Steps, vectors, and expression host are tailored to your specific target, not a generic template.
Run It
A clear, step-by-step protocol your team can take straight to the lab — and iterate on.
Example · Generated Protocol
In Detail
Everything a Bench Run Produces
A protocol is only useful if it is specific, sourced, and yours to change. Here is what each run gives you.
Protocol Stages
- Expression
- Purification
- Crystallization
- Cryo-EM
- Optimisation
The Generated Experiment
- A System-Generated First Draft
- A Potential-Success and Complexity Read
- Iteration History Kept per Run
- Refine Any Step in Place
Expected Outcomes
- Predicted Yield Range, in mg/L Culture
- Soluble Fraction
- Expression Time
- Harvest OD600
Construct Design
- Vector Suggestion
- Expression System and Strain
- Tag, Fusion, and Cleavage-Site Layout
- The Rationale Behind Each Domain Boundary
Literature Grounding
- Searches What Has Worked on Similar Targets and Hosts
- Cites Its Sources, So You Can Check Them
- Adapted to Your Target, Not a Generic Template
Handoff & Control
- Carries Picks Straight from Design and Stabilize
- Approve Each Stage Before the Next Opens
- Export the Protocol for Your Team
- Thumbs Up or Down on Every Generated Step
Bench writes protocols for your team to run. Orbion is fully computational and operates no wet lab of its own.
Take It to the Bench
Turn your top-ranked picks into a literature-grounded protocol adapted to your target and host — so the in-silico work actually reaches the lab.